gamma-tubulin and the C-terminal motor domain kinesin-like protein, KLPA, function in the establishment of spindle bipolarity in Aspergillus nidulans

Previous research has found that a gamma -tubulin mutation in Schizosacchar omyces pombe is synthetically lethal with a deletion of the C-terminal. mot or domain kinesin-like protein gene pk11, but the lethality of the double m utant prevents a phenotypic analysis of the synthetic interaction. We have investigated interactions between klpA1, a deletion of an Aspergillus nidul ans homolog of pk11, and mutations in the mipA, gamma -tubulin gene. We fin d that klpA1 dramatically increases the cold sensitivity and slightly reduc es the growth rate at all temperatures, of three mipA alleles. In synchroni zed cells we find that klpA1 causes a substantial but transient inhibition of the establishment of spindle bipolarity. At a restrictive temperature, m ipAD123 causes a slight, transient inhibition of spindle bipolarity and a m ore significant inhibition of anaphase A. In the mipAD123/klpA1 strain, for mation of bipolar spindles is more strongly inhibited than in the klpA1 sin gle mutant and many spindles apparently never become bipolar. These results indicate, surprisingly, that,tubulin and the klpA kinesin have overlapping roles in the establishment of spindle bipolarity. We propose a model to ac count for these data.