The human genome sequence provides the framework for understanding the biol
ogy of human cell function. The next step is to intensify the investigation
of protein function in the context of complex biological systems. Cellular
functions are carried out by molecular complexes acting in concert rather
than by single molecules or single reactions. Parallels have been drawn bet
ween scale-free nonbiologic networks and functionally interconnected metabo
lic pathways in the cell. Modeling of metabolic networks, in which function
al modules or subnetworks represent individual related pathways, will lead
to the prediction of protein function in the larger context of a complex sy
stem. Depending on the robustness of these metabolic networks, single-gene
defects alone or in combination with other gene defects and the environment
have the potential for invoking a spectrum of alterations in the integrity
of a given network. The overall purpose of this review is to highlight the
importance of simple heterozygosity for one pathogenic mutation or combina
torial heterozygosity for two or more mutations within or between individua
l genes in altering the stability of metabolic networks. Several forms of h
eterozygosity are considered, e.g., intra- and interallelic heterozygosity
and double heterozygosity. The concepts of synergistic heterozygosity, loss
of heterozygosity, and mitochondrial DNA heteroplasmy also are discussed i
n relation to the quantitative effects of coexisting mutations on the pheno
typic expression of disease. (C) 2001 Academic Press.