The density of corn pollen on leaves of milkweed plants inside and outside
of cornfields was measured in several studies from different localities. Th
e purpose was to obtain a representative picture of naturally occurring pol
len densities to provide a perspective for laboratory and field studies of
monarch larvae feeding on milkweed leaves with Bt corn pollen. Pollen densi
ty was highest (average 170.6 grains per cm(2)) inside the cornfield and wa
s progressively lower from the field edge outward, failing to 14.2 grains p
er cm(2) at 2 m. Inside the cornfield, and for each distance from the field
edge, a frequency distribution is presented showing the proportion of leaf
samples with different pollen densities. Inside cornfields, 95% of leaf sa
mples had pollen densities below 600 grains per cm(2) and the highest polle
n density observed was 1400 grains per cm(2), which occurred in a study wit
h a rainless anthesis period. All other studies had rainfall events during
the anthesis period. A single rain event can remove 54-86% of the pollen on
leaves. Leaves on the upper portion of milkweed plants, where young monarc
h larvae tend to feed, had only 30-50% of the pollen density levels of midd
le leaves.