Homology modelling of the ligand-binding domain of glucocorticoid receptor: binding site interactions with cortisol and corticosterone

Glucocorticoids are involved in the growth, development and homeostasis of a number of tissues. The physiological effects of this class of lipophilic steroids are mediated by ligand-inducible nuclear transcription factor, the glucocorticoid receptor/mineralocorticoid receptor, a member of the steroi d/nuclear receptor superfamily. The glucocorticoid receptor interacts speci fically with glucocorticoids, whereas the mineralocorticoid receptor intera cts with both glucocorticoids and mineralocorticoids. The molecular structu re of progesterone complexed to its receptor obtained from X-ray crystal st ructure analysis is used to build up a homology model of mouse glucocortico id receptor ligand-binding domain (mGR LBD). The secondary structure of mGR LBD contains 11 helices, nine turns and four sheets. The mGR LBD contains a long helix, H9, with 30 residues, and exhibits slight deformation when th e receptor protein binds with its cognate ligands. The mGR LBD has a 12-res idue C-terminal extension (residues 772-783) that is essential for hormone binding. This extension is tightly fixed in position by an antiparallel bet a -sheet interaction between amino acids 680-682 (S3) and 775-777 (S4). The three-dimensional model reveals two polar sites located at the extremities of the elongated hydrophobic ligand-binding pocket. Cortisol and corticost erone are docked to this ligand-binding pocket. The difference accessible s urface area study revealed the steroid-binding region of mGR LBD.