Surface properties of a specifically modified high-grade medical polyurethane

A high-grade medical polyurethane (PUR) was specifically modified to mimic the vascular vessel lumen. Since vascular endothelium represents a unique n on-thrombogenic surface, we developed a surface modification process to des ign a new PUR surface which promotes endothelial cell adhesion. Biologicall y active synthetic RGD-containing peptide has been covalently coupled on th e PUR surface. In order to optimise the RGD coupling, intermediate steps of PUR surface modification, such as plasma functionalisation and spacer poly saccharide grafting were investigated. Surface topography and friction imag es. chemistry and wettability differences of individual modification steps were controlled using atomic and lateral force microscopy, angle-resolved X -ray photoelectron spectroscopy and static contact angle measurements. Huma n umbilical vein endothelial cells adhesion tests were performed in vitro o n all the samples. Only the RGD-containing peptide-grafted PUR has shown th e endothelial cell attachment with an almost entire coverage of the surface substrate. (C) 2001 Elsevier Science B.V. All rights reserved.