Short communication: Erythrocyte binding antigen (EBA-175) of Plasmodium falciparum: improved genotype determination by nested polymerase chain reaction

Authors
Toure, FS Mavoungou, E Ndong, JMM Tshipamba, P Deloron, P
Citation
Fs. Toure et al., Short communication: Erythrocyte binding antigen (EBA-175) of Plasmodium falciparum: improved genotype determination by nested polymerase chain reaction, TR MED I H, 6(10), 2001, pp. 767-769
Citations number
16
Categorie Soggetti
Envirnomentale Medicine & Public Health
Journal title
TROPICAL MEDICINE & INTERNATIONAL HEALTH
ISSN journal
13602276 → ACNP
Volume
6
Issue
10
Year of publication
2001
Pages
767 - 769
Database
ISI
SICI code
1360-2276(200110)6:10<767:SCEBA(>2.0.ZU;2-K
Abstract
We have designed primers to the conserved region of the erythrocyte binding antigen (EBA)-175 gene which amplify specifically the two alleles by polym erase chain reaction (PCR) and by nested PCR. This approach provides a spec ific, sensitive and rapid method for genotype determination in a large numb er of Plasmodium falciparum field isolates.