Change in the degree of adsorption of proteins by aluminum-containing adjuvants following exposure to interstitial fluid: freshly prepared and aged model vaccines

The ability of interstitial fluid to change the degree of adsorption of ova lbumin to aluminum hydroxide adjuvant or lysozyme to aluminum phosphate adj uvant was studied. Ovalbumin and lysozyme were almost completely eluted aft er exposure at 37 degreesC to sheep lymph fluid for 4h or 15 min, respectiv ely. The ability of sheep lymph fluid to elute lysozyme from aluminum phosp hate adjuvant did not change as the,ed. However, only 60% of the ovalbumin adsorbed to aluminum hydroxide adjuvant was eluted during exposure to sheep model vaccine aged fluid for 24h after the model vaccine aged for 11 weeks at 4 degreesC. (C) 2001 Elsevier Science Ltd. All rights reserved.