Autologous platelet-rich plasma isolated using the Haemonetics Cell Saver 5 and Haemonetics MCS+ for the preparation of platelet gel

Background and Objectives We compared three methods of isolating platelet-r ich plasma (PRP) using the Haemonetics Cell Saver 5 and one method of isola ting PRP by plateletpheresis using the Haemonetics MCS+. PRP contains both platelets and fibrinogen, which are used in the preparation of haemostatic agents. Materials and Methods When the Haemonetics Cell Saver 5 was used, 500 ml of blood from each of 30 normal volunteer donors was collected into 70 ml of citrate-phosphate-dextrose (CPD) anticoagulant. In a further 14 normal volu nteers, the Haemonetics MCS+ was used to isolate PRP by plateletpheresis us ing an acid citrate dextrose (ACD) to blood ratio of 1 : 9. In a separate s tudy, CPD-anticoagulated whole blood from another 30 volunteers was used fo r measurement of fibrinogen levels in the plasma and cryoprecipitate. Results, A larger volume of PRP can be collected using the Haemonetics Cell .Saver 5 than by using the Haemonetics MCS+. The platelet concentration and the total number of platelets were higher in the PRP isolated using the Ha emonetics MCS+ than in the PRP isolated by the three methods used with the Haemonetics Cell Saver 5, with differences in platelet concentration and PR P volume among the four methods. The mean fibrinogen level in the plasma wa s 253 mg % +/- 47 (SD) and in the, cryoprecipitate, was 1085 mg O% +/- 304 (SD). Conclusions The, most appropriate method of PRP isolation for preparation o f platelet gel is dependent upon the specific surgical procedure to be unde rtaken and the patient's needs.