Acid protease purification by dye affinity chromatography

Authors
del Canizo, AAN Lahore, HMF Miranda, MV Cascone, O
Citation
Aan. Del Canizo et al., Acid protease purification by dye affinity chromatography, AFINIDAD, 58(493), 2001, pp. 231-233
Citations number
14
Categorie Soggetti
Chemistry
Journal title
AFINIDAD
ISSN journal
00019704 → ACNP
Volume
58
Issue
493
Year of publication
2001
Pages
231 - 233
Database
ISI
SICI code
0001-9704(200105/06)58:493<231:APPBDA>2.0.ZU;2-#
Abstract
After screening of 9 immobilised triazinic dyes, Red F5B was selected as th e best ligand for chymosin and Mucor miehei acid protease purification, at pHs 6.5 and 5.4 respectively. For Mucor bacilliformis protease purification from a crude extract of a solid-state culture, Red HE-3B was selected, all owing a 95.2% recovery of pure enzyme.