Chromate reduction and 16S rRNA identification of bacteria isolated from aCr(VI)-contaminated site

A Gram-positive, hexavalent chromium [chromate: Cr(Vl)]-tolerant bacterium. isolated from tannery waste from Pakistan, was identified as a Microbacter ium sp. by 16S rRNA gene sequence homology. The strain (designated as MP30) reduced toxic Cr(VI) only under anaerobic conditions at the expense of ace tate as the electron donor. The bacterium was able to grow aerobically in L -broth supplemented with 15 mM CrO42- but then did not reduce Cr(VI). At a concentration of 2.4X10(9) cells/ml, 100 muM sodium chromate was reduced wi thin 30 h; however, the maximum specific reduction rate was obtained at low er initial cell concentrations.