Monitoring nuclear import with GFP-variant fusion proteins in digitonin-permeabilized cells

Authors
Petrulis, JR Perdew, GH
Citation
Jr. Petrulis et Gh. Perdew, Monitoring nuclear import with GFP-variant fusion proteins in digitonin-permeabilized cells, BIOTECHNIQU, 31(4), 2001, pp. 772-775
Citations number
4
Categorie Soggetti
Biochemistry & Biophysics
Journal title
BIOTECHNIQUES
ISSN journal
07366205 → ACNP
Volume
31
Issue
4
Year of publication
2001
Pages
772 - 775
Database
ISI
SICI code
0736-6205(200110)31:4<772:MNIWGF>2.0.ZU;2-F
Abstract
A convenient assay for monitoring nuclear localization signal-mediated nucl ear import of green fluorescent protein (GFP)-variant fusion proteins has b een developed. This modified assay relies upon indirect immunofluorescence microscopy for visualization of transported substrates. The use of GFP-vari ant fusion proteins allows for the rapid assessment of optimal digitonin co ncentration and permits nuclear import to be monitored with minimal sample preparation in real time.