Detection of cytochrome P450 mRNA transcripts in prostate samples by RT-PCR

Background The expression of cytochrome P450 (CYP)-dependent mono-oxygenase s in the prostate is important, as it will determine the rate of activation of potential carcinogens as well as the metabolism of hormones with implic ations in diseases of the prostate. In addition, the levels of cytochromes P450 in prostatic tumours may well be determinants of the outcome of therap y involving P450 substrates such as anti-androgens. Methods The gene expression of 12 different CYP genes was measured by rever se transcription-polymerase chain reaction (RT-PCR) in a total of 28 human prostatic tumour and nontumour samples. Results Intriguingly, a large number of CYP mRNAs were detected in the pros tate samples, including CYP1A2, -1B1, -2C19, -2D6, -3A4, -3A5, -3A7 and -4B 1. CYP1B1 was consistently expressed and CYP3A5 and CYP4B1 were expressed i n a majority of the samples tested. Conclusions These data demonstrate a wide range of CYP genes being expresse d in the prostate. The relative importance of these enzymes in the pathogen esis and treatment of prostatic disease remains an important theme for furt her study.