Lentiviral vectors show dramatically increased efficiency of transduction of human leukemic cell lines

Authors
Bambacioni, F Casati, C Serafini, M Manganini, M Golay, J Introna, M
Citation
F. Bambacioni et al., Lentiviral vectors show dramatically increased efficiency of transduction of human leukemic cell lines, HAEMATOLOG, 86(10), 2001, pp. 1095-1096
Citations number
10
Categorie Soggetti
Cardiovascular & Hematology Research
Journal title
HAEMATOLOGICA
ISSN journal
03906078 → ACNP
Volume
86
Issue
10
Year of publication
2001
Pages
1095 - 1096
Database
ISI
SICI code
0390-6078(200110)86:10<1095:LVSDIE>2.0.ZU;2-I
Abstract
Moloney murine leukemia virus (MMLV)-based vectors have been widely used in vitro and in clinical studies. Subsequently, HIV-1 derived lentivectors we re introduced(1) in which the env, vif vpr, vpu and nef genes are deleted.( 2) Another version of the lentivector has been developed which contains a 1 18 bp non-coding sequence from the HIV-1 pol gene.(3,4) We compare the capa city of these different retro-viruses(5-7) to transduce human hematopoietic lines.