Background/Aims: It was generally believed, but not proved, that early cirr
hosis may be reversible, while advanced cirrhosis may not. This present stu
dy is to compare in mice the spontaneous regression of liver fibrosis betwe
en early and more advanced stage.
Methodology: Liver fibrosis in mice was induced by intraperitoneal. injecti
on of carbon tetrachloride for 4, 10, and 16 weeks. After the last dose of
each schedule, mice were sacrificed 1 day later (progression model) or left
untreated for 10, 20, and 60 days (regression model). Tissue sections were
stained by Sirius red. Liver hydroxyproline levels were determined to asse
ss severity of fibrosis. Gelatinases in tissue extracts were assayed by zym
ography.
Results: During regression, diminution of fibrotic bands was more prominent
in the 4-week group than in the others. Liver hydroxyproline levels in the
progression model increased and resolution of liver fibrosis in the regres
sion model decreased as carbon tetrachloride injection was prolonged. Liver
matrix metalloproteinase-2 and -9 activities in the progression model also
decreased as the injection was prolonged.
Conclusions: These data demonstrated that reversibility of liver fibrosis w
ould be gradually lost as liver injuries were prolonged. Gradual loss of th
e expression of matrix metalloproteinases may be responsible for the loss o
f reversibility.