DNA biosensors are realised immobilising a DNA structure on a suitable tran
sducer to obtain selective information. In this paper we show how the deter
mination of low-molecular weight compounds with affinity for DNA was measur
ed by their effect on the oxidation signal of the guanine peak of calf thym
us DNA immobilised on the electrode sensor and investigated by chronopotent
iometric analysis. The DNA biosensor is able to detect known intercalating
and groove binding compounds. Applicability to river water samples was demo
nstrated.
Moreover, a piezoelectric sensor coupled to a short oligonucleotide can be
used as detector of the hybridisation reaction. We show as a model the dete
ction of a specific mutation in apolipoprotein E (ApoE) gene.
Biotinylated 23-mer probes were immobilised on the streptavidin coated gold
surface of a quartz crystal; the protein was covalently bound to the thiol
/dextran modified gold surface. The device was able to distinguish differen
t synthetic oligonucleotides. The hybridisation reaction was also performed
using real samples of DNA extracted from human blood and amplified by Poly
merase chain reaction PCR, The extension of such procedure to samples of en
vironmental interest is discussed.