Potentialities of quantitative analysis by high-performance liquid chromatography without reference standards of analytes: Use of reference data on UV spectra for calculating calibration coefficients

It was shown by the examples of simple organic compounds and some medicinal substances that, in the quantitative analysis by the internal standard met hod in high-performance liquid chromatography (HPLC) with UV detection, one can use calibration coefficients calculated from reference data on UV spec tra. Determinations should be performed at several constant wavelengths (fo r example, 220, 254, 280, and 320 nm) using at least two internal standards (a set of eight samples was proposed) rather than one standard. As can be judged from the estimates obtained, the systematic component of the determi nation error under these conditions does not exceed the contributions of ra ndom errors. The developed approach opens up new opportunities for quantita tive determinations by HPLC without reference samples of analytes, instead of which reference data on molar absorptivities are used.