On complex I and other NADH : Ubiquinone reductases of Neurospora crassa mitochondria

Citation
A. Videira et M. Duarte, On complex I and other NADH : Ubiquinone reductases of Neurospora crassa mitochondria, J BIOENER B, 33(3), 2001, pp. 197-203
Citations number
78
Categorie Soggetti
Biochemistry & Biophysics
Journal title
JOURNAL OF BIOENERGETICS AND BIOMEMBRANES
ISSN journal
0145479X → ACNP
Volume
33
Issue
3
Year of publication
2001
Pages
197 - 203
Database
ISI
SICI code
0145-479X(200106)33:3<197:OCIAON>2.0.ZU;2-N
Abstract
The mitochondrial complex I is the first component of the respiratory chain coupling electron transfer from NADH to ubiquinone to proton translocation across the inner membrane of the organelle. The enzyme from the fungus Neu rospora crassa is similar to that of other organisms in terms of protein an d prosthetic group composition, structure, and function. It contains a high number of polypeptide subunits of dual genetic origin. Most of its subunit s were cloned, including those binding redox groups. Extensive gene disrupt ion experiments were conducted, revealing many aspects of the structure, fu nction, and biogenesis of complex I. Complex I is essential for the sexual phase of the life cycle of N. crassa, but not for the asexual stage. In add ition to complex I, the fungal mitochondria contain at least three nonproto n-pumping alternative NAD(P)H dehydrogenases feeding electrons to the respi ratory chain from either matrix or cytosolic substrates.