In vitro and in vivo analysis of the immune system of vitamin D receptor knockout mice

Immune cells carry receptors for 1,25-dihydroxyvitamin D-3 [1,25(OH)(2)D-3; vitamin D receptor (VDR)] and individuals with severe vitamin D deficiency have immune abnormalities. The aim of this study was to investigate the ro le of vitamin D in the immune system by studying VDR-knockout (VDR-KO) mice . VDR-KO mice had the same metabolic phenotype as rachitic animals with sev ere hypocalcemia. Leukocytosis, lymphocyte subset composition in different immune organs, and splenocyte proliferation to several stimuli were normal, except for a lower response to anti-CD3 stimulation (simulation index [SI] of 13 +/- 4 vs. 24 +/- 9 in wild-type mice; p < 0.01). Macrophage chemotax is was impaired (41 +/- 19% vs. 60 +/- 18% in wild-type mice; p < 0.01) but phagocytosis and killing were normal. In vivo rejection of allogeneic (31 +/- 12 days vs. 45 +/- 26 days of survival in wild-type mice, NS) or xenoge neic (10 +/- 2 days vs. 16 +/- 9 days of survival in wild-type mice, NS) is let grafts was comparable with wild-type mice. Surprisingly, VDR-KO mice we re protected from low-dose streptozotocin-induced diabetes mellitus (LDSDM; 5% vs. 65% in wild-type mice; p < 0.001). Correcting hypocalcemia by use o f lactose-rich or polyunsaturated fat-rich diets fully restored the immune abnormalities in vitro and the sensitivity to diabetes in vivo. On the othe r hand, treatment with 1,25(OH)(2)D-3 protected wild-type mice against diab etes but did not protect normocalcemic VDR-KO mice. We conclude that immune defects observed in VDR-KO mice are an indirect consequence of VDR disrupt ion because they can be restored by calcium homeostasis normalization. This study proves that although 1,25(OH)(2)D-3 is a pharmacologic and probably a physiological immunomodulator, its immune function is redundant. Moreover , we confirm the essential role of calcium in the immune system.