We determine the non-abelian Born-Infeld action, including fermions, as it
results from the four-point tree-level open superstring scattering amplitud
es at order alpha'(2). We find that, after an appropriate field redefinitio
n all terms at this order can be written as a symmetrised trace. We confron
t this action with the results that follow from kappa-symmetry and conclude
that the recently proposed non-abelian kappa-symmetry cannot be extended t
o cubic orders in the Born-Infeld curvature.