DNA VACCINATION AND GENE-THERAPY BASED ON TRANSIENT EXPRESSION OF NUCLEIC-ACIDS IN HUMAN AND ANIMAL SOMATIC-CELLS

DNA of plasmids and viruses deficient in replication in animal and hum an cells is preserved in the cel for a long time without being integra ted in the genome, and governs synthesis of the encoded proteins for w eeks and months. The same applies to RNA-containing viruses. Such prol onged transient expression combined with improved techniques of DNA an d RNA transfer to animal and human cells and organs in vivo become the basis for gene therapy and new-generation vaccines.