Sensitive LC determination of piroxicam after in vitro transdermal permeation studies

A direct, very sensitive. simple and rapid high-performance liquid chromato graphic (HPLC) method for the determination of piroxicam, with tenoxicam as internal standard, has been developed and validated. Samples were chromato graphed on a 5 mum Scharlau C-18 column. The mobile phase was a mixture of acetonitrile-acetic acid 4% (pH 2.8) (45:55, v/v). Detection was at 354 run and the run time was 7 min. The limit of detection was 0.025 mug/ml. The d etector response was found to be linear in the concentration range 0.05-9 m ug/ml. This HPLC assay has been applied to measure the 'in vitro' percutane ous permeation of piroxicam through abdominal hairless rat skin, using Fran z-type diffusion cells, in order to obtain the concentration-time profiles of piroxicam. (C) 2001 Elsevier Science B.V. All rights reserved.