LC determination of carbamazepine in murine brain

A reversed phase HPLC method for the determination of carbamazepine (CBZ) i n the brain of adult mice is described. CBZ was recovered from murine brain by solvent-extraction with ethyl acetate and resolved from imipramine (int ernal standard) and brain endogenous material using a Lichrospher RP select B column with a linear gradient of acetonitrile (40 80 v/v, 25 min) in amm onium acetate buffer (25 mM, pH 4.0) with UV detection at 285 nm. The metho d is selective, reproducible and precise with a limit detection of 45 ng/ml and is suitable for the determination of CBZ in murine brain after intra-p eritoneal administration. (C) 2001 Elsevier Science B.V. All rights reserve d.