Enzyme-assisted aqueous extraction of rice bran oil

In the present study, rice bran oil was extracted by enzyme-assisted aqueou s extraction under optimized aqueous extraction conditions using mixtures o f Protizyme((TM)) (protease; Jaysons Agritech Pvt. Ltd., Mysore, India), Pa lkodex((TM)) (alpha -amylase; Maps India Ltd., Ahmedabad, India), and cellu lase (crude cellulase; Central Drug House, Delhi, India). The optimal condi tions used were: mixtures of amylase (80 U), protease (368 U), and cellulas e (380 U), with 10 g of rice bran in 40 mL distilled water, pH 7.0, tempera ture 65 degreesC, extraction time 18 h with constant shaking at 80 rpm. Cen trifugation of the mixture at 10,000 x g for 20 min yielded a 77% recovery of the oil.