Sequences adjacent to oriP improve the persistence of Epstein-Barr virus-based episomes in B cells

Authors
White, RE Wade-Martins, R James, MR
Citation
Re. White et al., Sequences adjacent to oriP improve the persistence of Epstein-Barr virus-based episomes in B cells, J VIROLOGY, 75(22), 2001, pp. 11249-11252
Citations number
20
Categorie Soggetti
Microbiology
Journal title
JOURNAL OF VIROLOGY
ISSN journal
0022538X → ACNP
Volume
75
Issue
22
Year of publication
2001
Pages
11249 - 11252
Database
ISI
SICI code
0022-538X(200111)75:22<11249:SATOIT>2.0.ZU;2-B
Abstract
Epstein-Barr virus (EBV) oriP and the EBV nuclear antigen 1 (EBNA-1) protei n allow persistence of EBV-based episomes. A nuclear matrix attachment regi on (MAR) spans oriP and the adjacent region of the EBV genome containing th e EBV-expressed RNAs. Here, we show that episomes with the MAR are retained significantly more efficiently in EBV-positive B cells than episomes conta ining oriP alone.