F. Minibayeva et Rp. Beckett, High rates of extracellular superoxide production in bryophytes and lichens, and an oxidative burst in response to rehydration following desiccation, NEW PHYTOL, 152(2), 2001, pp. 333-341
. The mechanism of extracellular superoxide radical (O-2(.-),) formation an
d the role of the oxidative burst in response to desiccation stress is repo
rted here in bryophytes and lichens from habitats of contrasting water avai
lability. .
. Rates of extracellular production of O-2(.-) radicals were measured, befo
re and after desiccation stress, by the oxidation of epinephrine to adrenoc
hrome, determined spectrophotometrically.
. Several desiccation-sensitive lichens and bryophytes that grow in very we
t microhabitats produced O-2(.-) extracellularly at high rates, even when t
hey were not stressed. In addition, some species showed a powerful burst of
O-2(.-) production during rehydration following desiccation. Production of
high levels of O-2(.-) and the existence of an inducible oxidative burst w
as best developed in cyanobacterial lichens, a hornwort and the two thalloi
d liverworts tested. Extracellular production of O-2(.-) was almost absent
from all mosses tested, a leafy liverwort, a filmy fern and from desiccatio
n-tolerant lichens.
. Patterns of O-2(.-) production are discussed in terms of their possible r
ole as a defence against pathogenic fungi and bacteria.