J. Alfonso et al., WHEAT AND BARLEY INHIBITORS ACTIVE TOWARDS ALPHA-AMYLASE AND TRYPSIN-LIKE ACTIVITIES FROM SPODOPTERA-FRUGIPERDA, Journal of chemical ecology, 23(7), 1997, pp. 1729-1741
The alpha-amylase activity was determined throughout the larval develo
pment of Spodoptera frugiperda. Maximal activities with optimal pH in
the range 8.5-9.5 were found in last instars. Protein preparations enr
iched in heterotetrameric inhibitors from wheat flour were active towa
rds gut amylases from last instars, while those corresponding to homod
imeric and monomeric inhibitors showed low inhibition levels. These re
sults were further supported by testing purified members of each inhib
itor type and by analyzing the effects of the inhibitors on the amylas
e isoenzyme pattern from native PAGE. High levels of trypsin-like acti
vity were also found in gut extracts from last instars. Different gene
tic variants of the major barley trypsin inhibitor were active against
this gut enzyme. None of the other larval digestive protease activiti
es (chymotrypsin-like, elastase-like, leucine aminopeptidase-like, and
carboxypeptidase A and B-like) were inhibited, indicating that the ba
rley inhibitor is specific towards trypsin-like enzymes.