Two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) continues to d
eliver high quality protein resolution and dynamic range for the proteomics
researcher. To remain as the preferred method for protein separation and c
haracterization, several key steps need to be implemented to ensure quality
sample preparation and speed of analysis. Here, we describe the progress m
ade towards establishing 2D-PAGE as the optimal separation tool for proteom
ics research.