Selection of autochthonous yeast strains able to degrade biphenyl

Citation
Mc. Romero et al., Selection of autochthonous yeast strains able to degrade biphenyl, WORLD J MIC, 17(6), 2001, pp. 591-594
Citations number
17
Categorie Soggetti
Biotecnology & Applied Microbiology
Journal title
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
ISSN journal
09593993 → ACNP
Volume
17
Issue
6
Year of publication
2001
Pages
591 - 594
Database
ISI
SICI code
0959-3993(2001)17:6<591:SOAYSA>2.0.ZU;2-4
Abstract
In order to assess the role of yeasts in the natural detoxification process of sediments polluted with biaryl compounds, indigenous yeast species able to degrade biphenyl (BP) were isolated and identified. The degradation abi lity of 24 strains of the genera Candida spp., Cryptococcus spp., Pichia sp p., Rhodotorula spp., Trichosporon spp. and Yarrowia spp. was evaluated by the identification of the BP-metabolites, by HPLC analysis. 4-Hydroxybiphen yl was the main derivative in the Candida krusei, C. tenuis, C. tropicalis, Pichia haplophila, Rhodotorula glutinis, Trichosporon pullulans and Yarrow ia lipolytica cultures. 3-Hydroxybiphenyl was detected in minor amounts in the culture supernatant of C. tropicalis, C. krusei strains and R. glutinis . Further hydroxylation led to 3,4-dihydroxy and 2,3-dihydroxybiphenyl; the former in C. tropicalis, C. krusei and R. glutinis cultures, and the latte r only in the R. glutinis assays. The cleavage product 4-phenyl-2-pyrone-6- carboxylic acid, was observed in R. glutinis and Y. lipolytica cultures. Th e degradation ability of the R. glutinis isolates was noteworthy; as four h ydrolxylated intermediates and a ring-cleavage product were obtained in bot h strain cultures. The species studied in this report were dominant in poll uted sediments; furthermore, R. glutinis had been mentioned as able to degr ade other aromatic hydrocarbons and had high relevance in bioremediation ex periments.