Human pancreatic glucokinase (GlkB) complements the glucose signalling defect of Saccharomyces cerevisiae hxk2 mutants

Authors
Mayordomo, I Sanz, P
Citation
I. Mayordomo et P. Sanz, Human pancreatic glucokinase (GlkB) complements the glucose signalling defect of Saccharomyces cerevisiae hxk2 mutants, YEAST, 18(14), 2001, pp. 1309-1316
Citations number
34
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
YEAST
ISSN journal
0749503X → ACNP
Volume
18
Issue
14
Year of publication
2001
Pages
1309 - 1316
Database
ISI
SICI code
0749-503X(200110)18:14<1309:HPG(CT>2.0.ZU;2-1
Abstract
Human pancreatic glucokinase (GlkB, hexokinase IV) has been expressed in Sa ccharomyces cerevisiae. The recombinant protein showed similar enzyme kinet ics to those described for the original enzyme. When expressed in hxk2 yeas t mutants, GlkB complemented both the glucose induction and the glucose rep ression defects present in the mutant. It was also functional in regulating the activity of the Snf1 kinase complex in response to glucose, participat ing in the regulation of the Reg1/Glc7 phosphatase complex, as its yeast co unterpart. Copyright (C) 2001 John Wiley & Sons, Ltd.