Pk. Bansal et al., A minisatellite sequence in the upstream region of the DURA3 gene from thehalotolerant yeast Debaryomyces hansenii, YEAST, 18(14), 2001, pp. 1301-1307
The URA3 gene of Deharyomyces hansenii, encoding orotidine 5'-phosphate dec
arboxylase enzyme, was isolated by complementation in the yeast Saccharomyc
es cerevisiae. The deduced amino acid sequence is highly similar to Ura3 pr
oteins from other yeast and fungal species. Analysis of the region upstream
of the coding sequence revealed the presence of AG-rich minisatellite DNA
sequences. In addition, upstream of the DURA3 sequence, we have found the 3
'-terminal of a gene encoding a GEA2-like protein. The GenBank Accession No
. of this sequence is AY033329. Copyright (C) 2001 John Wiley & Sons, Ltd.