Jm. Pascal et al., Mouse testis-brain RNA-binding protein (TB-RBP): expression, purification and crystal X-ray diffraction, ACT CRYST D, 57, 2001, pp. 1692-1694
TB-RBP (testis-brain RNA-binding protein) is a mouse RNA-binding protein th
at controls the temporal and spatial expression of mRNA. Found most abundan
tly in brain and male germ cells in the testis, TB-RBP is known to suppress
the translation of specific testicular and brain mRNAs as part of cell dev
elopment. TB-RBP- mRNA complexes can bind microtubules and thereby facilita
te RNA translocation. Translin is the human orthologue of TB-RBP which bind
s to single-stranded ends of DNA sequences in breakpoint regions of chromos
omal translocations. TB-RBP/translin has been crystallized in space group P
2(1)2(1)2. The expression, purification, and crystallization of TB-RBP are
described as well as preliminary X-ray diffraction data. The multimeric sta
te of TB-RBP is addressed using dynamic light-scattering results.