PEROXIDASE-RELEASE ASSOCIATED WITH PHAGOCYTOSIS IN MYTILUS-GALLOPROVINCIALIS HEMOCYTES

Fluorescence spectra of the supernatant of adherent haemocyte monolaye rs from Mytilus galloprovincialis, supplemented with homovanillic acid or with a tyrosyl peptide glycylglycyltyrosine (GGY), were recorded b efore and after stimulation by zymosan. The formation of fluorescent d erivatives was observed to have spectral characteristics similar to th ose of fluorescent compounds generated by the exposure of homovanillic acid or GGY to a horseradish peroxidase/hydrogen peroxide system in v itro. Lucigenine-enhanced chemiluminescence (CLluc) of M. galloprovinc ialis haemocytes stimulated by zymosan or by phorbol ester (PMA) was m easured in the presence and absence of sodium azide, a peroxidase inhi bitor. Sodium azide inhibited the CLluc of haemocytes stimulated by zy mosan, an effective stimulus for myeloperoxidase secretion in human po lymorphonuclear leukocytes, but not the CLluc of haemocytes stimulated by PMA, indicating the presence of peroxidases with some properties o f myeloperoxidase, in adherent haemocytes from M. galloprovincialis. ( C) 1997 Elsevier Science Ltd.