Experimental infection of cats with Tritrichomonas foetus

Objective-To determine whether infection with Tritrichomonas foetus causes diarrhea in specific-pathogen-free or Cryptosporidium coinfected cats. Animals-4 cats with subclinical cryptosporidiosis (group 1) and 4 specific- pathogen-free cats (group 2). Procedure-Cats were infected orogastrically with an axenic culture of T foe tus isolated from a kitten with diarrhea. Direct microscopy and protozoal c ulture of feces, fecal character, serial colonic mucosal biopsy specimens, and response to treatment with nitazoxanide (NTZ; group 1) or prednisolone (groups 1 and 2) were assessed. Results-Infection with T foetus persisted in all cats for the entire 203-da y study and resulted in diarrhea that resolved after 7 weeks, Group-1 cats had an earlier onset, more severe diarrhea, and increased number of trichom onads on direct fecal examination, compared with group-2 cats. Use of NTZ e liminated shedding of T foetus and Cryptosporidium oocysts, but diarrhea co nsisting of trichomonad-containing feces recurred when treatment was discon tinued. Prednisolone did not have an effect on infection with T foetus but resulted in reappearance of Cryptosporidium oocysts in the feces of 2 of 4 cats. During necropsy, T foetus was isolated from contents of the ileum, ce cum, and colon. Tritrichomonas foetus organisms and antigen were detected o n surface epithelia and within superficial detritus of the cecal and coloni c mucosa. Conclusions and Clinical Relevance-After experimental inoculation in cats, T foetus organisms colonize the ileum, cecum, and colon, reside in close co ntact with the epithelium, and are associated with transient diarrhea that is exacerbated by coexisting cryptosporidiosis but not treatment with predn isolone.