Characterization of two new alleles at the goat CSN1S2 locus

Two novel alleles at the goat CSN1S2 locus have been identified: CSN1S2(F) and CSN1S2(D). Sequence analyses revealed that the CSN1S2(F) allele is char acterized by a G -->A transition at the 13th nucleotide in exon 3 changing the seventh amino acid of the mature protein from Val to Ile. The CSN1S2(D) allele, apparently associated with a decreased synthesis of alpha s2-casei n. is characterized by a 106-bp deletion. involving the last 11 bp of the e xon 11 and the first 95 bp of the following intron. Methods (PCR-RFLP and P CR) for identification of carriers of these alleles have been developed.