Mapping of 12 bovine ribosomal protein genes using a bovine radiation hybrid panel

Twelve bovine ribosomal protein genes, for which sequence data had been acq uired from complementary deoxyribonucleic acid (cDNA) clones isolated from a cattle skin cDNA library. were mapped. As ribosomal protein genes are a g roup of highly conserved house keeping genes, specific primers were designe d to span the intron-exon splice sites and to amplify intronic sequences, i n order to obtain bovine-specific polymerase chain reaction (PCR) products. Two of 12 ribosomal protein genes were genotyped in this way and the remai ning 10 were mapped using additional primers designed from within the intro n. Eleven previously unmapped ribosomal protein genes were localized and on e previously reported ribosomal protein gene localization was confirmed. Th e 12 ribosomal protein genes mapped in this study are spread over 10 chromo somes, including the X chromosome. The locations show conservation of compa rative map position in cattle and human.