Isolation of expressed sequence tags of skeletal muscle of neonatal healthy and splay leg piglets and mapping by somatic cell hybrid analysis

We have isolated 14 differentially displayed and 10 further expressed seque nce tags (ESTs) from Musculus biceps femoris of newborn healthy and splay l eg piglets. By comparison with EMBL/GenBank data we could identify nine por cine homologues to human genes (TATA box binding protein associated factor B TAF1B: B-cell CLL/lymphoma 7B BCL7B; pyruvate dehydrogenase kinase, isoen zyme 4 PDK4; ribosomal protein S10 RPS10; SPARC-like 1 SPARCL1; epithelial protein lost in neoplasm beta EPLIN; N-myc downstream-regulated gene 2 NDRG 2; pleiomorphic adenoma gene like 2 PLAGL and, BCL-2 associated transcripti on factor short form BTFS). Eight fragments correspond to uncharacterized E STs and 7 ESTs had no significant match with database sequences. These data provide the first expression profiles in skeletal muscle of neonatal pigle ts and are a basic for candidate gene investigations for congenital splay l eg in piglets, Eleven ESTs were physically mapped to porcine chromosomes 1, 3, 4, 5, 7, 8, 9 and 10 and contribute to the comparative map of humans an d pigs.