Mapping of rabbit chromosome 1 markers generated from a microsatellite-enriched chromosome-specific library

Authors
Korstanje, R Gillissen, GF den Bieman, MG Versteeg, SA van Oost, B Fox, RR van Lith, HA van Zutphen, LFM
Citation
R. Korstanje et al., Mapping of rabbit chromosome 1 markers generated from a microsatellite-enriched chromosome-specific library, ANIM GENET, 32(5), 2001, pp. 308-312
Citations number
13
Categorie Soggetti
Animal Sciences","Molecular Biology & Genetics
Journal title
ANIMAL GENETICS
ISSN journal
02689146 → ACNP
Volume
32
Issue
5
Year of publication
2001
Pages
308 - 312
Database
ISI
SICI code
0268-9146(200110)32:5<308:MORC1M>2.0.ZU;2-M
Abstract
A genomic DNA library was produced from flow-sorted rabbit chromosome I and enriched for fragments containing CA-repeats. Clones containing CA-repeats were identified and primers for amplification of the microsatellite were d eveloped after sequencing the clone. The degree of polymorphism was tested in rabbits from different breeds. This approach identified 12 microsatellit e markers which could be used for studying linkage relationships in the pro geny of an F-2-intercross: (AX/JUxIIIVO/JU) F-2, and two backcrosses: (OS/J xX/J)X/J and (WH/JxX/J)X/J. Seven of these markers were mapped on chromosom e 1.