R. Korstanje et al., Mapping of rabbit chromosome 1 markers generated from a microsatellite-enriched chromosome-specific library, ANIM GENET, 32(5), 2001, pp. 308-312
A genomic DNA library was produced from flow-sorted rabbit chromosome I and
enriched for fragments containing CA-repeats. Clones containing CA-repeats
were identified and primers for amplification of the microsatellite were d
eveloped after sequencing the clone. The degree of polymorphism was tested
in rabbits from different breeds. This approach identified 12 microsatellit
e markers which could be used for studying linkage relationships in the pro
geny of an F-2-intercross: (AX/JUxIIIVO/JU) F-2, and two backcrosses: (OS/J
xX/J)X/J and (WH/JxX/J)X/J. Seven of these markers were mapped on chromosom
e 1.