Comparative porcine gene mapping relative to human chromosomes 9, 10, 20 and 22

Comparative anchor tagged sequence (CATS) consensus primers from loci mappe d to human chromosomes 9, 10, 20, and 22 have been used to amplify homologo us loci in pigs. Of 53, CATS primers tested in pigs, only 23 yielded produc ts homologous to the human locus (42% success). Ten loci were physically ma pped (43% success rate for verified products, but only 19% for primers test ed). Due to lack of polymorphism, linkage mapping was possible only for AMB P. Map locations were consistent with human/pig ZOO-FISH, except for ADRA1A , whose position is still equivocal in humans. These CATS primers have made very limited contributions to pig/human comparative gene mapping because o f low efficiency of amplification of orthologous porcine product, frequent amplification from rodent template in a somatic hybrid panel and low level of polymorphism.