Production of native and recombinant lipases by Candida rugosa - A review

The yeast Candida rugosa produces multiple lipase isoenzymes sharing high s equence homology but with some differences in their catalytic properties. T he regulation of C. rugosa lipase (CRL) synthesis and secretion in C. rugos a obeys a complex pattern. Fermentation processes for both wildtype and mut ant C. rugosa strains are available for lipase production. Native CRL prepa rations have been extensively used for biotransformations. However, their i nherent mixture of isoforms with variable profiles complicates interpretati on and brings into question the reproducibility achieved between preparatio ns. Although heterologous CRLs gene expression had been hampered owing to a nonuniversal codon usage, recent advances have made heterologous CRLs avai lable. This will expand and improve the industrial utility of CRLs even fur ther. The purpose of this review is to provide a summary of the recent adva nces on the production of native and recombinant lipases by C. rugosa.