M. Prata-vidal et al., An experimental study of caseinomacropeptide hydrolysis by trypsin in a continuous membrane reactor, BIOCH ENG J, 8(3), 2001, pp. 195-202
Caseinomacropeptide (CMP) is the product of milk K-casein hydrolysis by chy
mosin. This macropeptide with a molecular weight of about 7000 g mol(-1) co
ntains several sequences of amino acids which show promising nutritional or
biological properties. We have previously reported the production of such
sequences peptides) from CMP by trypsin as well as the reaction mechanism i
nvolved. In this study, we have focused on the study of the hydrolytic reac
tion and on the retention by the membrane of the peptides generated by the
reaction, both in a batch system and in a continuous stirred reactor. Facto
rs influencing the operation of the membrane reactor such as the substrate
concentration, ionic strength, transmembrane pressure are studied and discu
ssed. Conclusions on the design of a membrane reactor are then drawn. (c) 2
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