Am. Calado et al., Stereologic characterization of bovine (Bos taurus) cumulus-oocyte complexes aspirated from small antral follicles during the diestrous phase, BIOL REPROD, 65(5), 2001, pp. 1383-1391
Bovine ovarian cumulus-oocyte complexes (COCs) are used for in vitro matura
tion and fertilization after selection by size and morphology, but their de
velopmental potential remains low. Stereology could provide more objective
criteria for selecting the most competent complexes, but its application is
lacking in cattle. COCs from small (1-4 mm) antral follicles were aspirate
d from diestrous ovaries of Holstein-Friesian cows, fixed in glutaraldehyde
, randomly embedded in glycol-methacrylate, and sectioned at 20 mum. The un
biased nucleator principle was used for estimating the mean volumes of comp
lexes, oocytes, cumulus cells, and nuclei of oocytes and cumulus cells. The
thickness of the zona pellucida and the relative numerical percentages of
the several morphologic types of cumulus cells were also evaluated. The opt
ical disector procedure was used for cumulus cell sampling. Volume estimati
on based on a real physical unique point did not differ from those based on
a particular point among many or on a virtual central point, and the mean
cumulus cell volume was estimated by using the single section bearing the u
nique reference point. Quantitative data showed that COCs appear heterogene
ous for all studied parameters and that the cumulus mass contains three dif
ferent cell populations.