Citation
Ng. Balabushevitch et al., Encapsulation of proteins by layer-by-layer adsorption of polyelectrolytesonto protein aggregates: Factors regulating the protein release, BIOTECH BIO, 76(3), 2001, pp. 207-213
Citations number
28
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
BIOTECHNOLOGY AND BIOENGINEERING
ISSN journal
00063592
→ ACNP
Volume
76
Issue
3
Year of publication
2001
Pages
207 - 213
Database
ISI
SICI code
0006-3592(200111)76:3<207:EOPBLA>2.0.ZU;2-K
Abstract
A novel method of protein encapsulation is proposed. Preformed protein aggr
egates are covered with polyelectrolyte layers by means of layer-by-layer a
dsorption. The polyelectrolyte membrane prevents protein leakage out of the
capsule. Using chymotrypsin as a model enzyme the capsule wall selective p
ermeability was demonstrated for substrates and inhibitors of different mol
ecular weight and solubility. (C) 2001 John Wiley & Sons, Inc.