Overproduction of pyoverdins by Fur mutants of Pseudomonas aeruginosa strains PAO1 and Fe10 in stirred bioreactors

Citation
A. Johnova et al., Overproduction of pyoverdins by Fur mutants of Pseudomonas aeruginosa strains PAO1 and Fe10 in stirred bioreactors, BIOTECH LET, 23(21), 2001, pp. 1759-1763
Citations number
15
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
BIOTECHNOLOGY LETTERS
ISSN journal
01415492 → ACNP
Volume
23
Issue
21
Year of publication
2001
Pages
1759 - 1763
Database
ISI
SICI code
0141-5492(2001)23:21<1759:OOPBFM>2.0.ZU;2-D
Abstract
Fur mutants FPA12 and FF13 of strains Pseudomonas aeruginosa PAO1 and Fe10, respectively, were prepared and their production of pyoverdin evaluated. T he strains were cultivated in stirred bioreactor in iron-deficient and iron -supplemented medium containing Casamino acids (CA) or succinate as a sourc e of carbon and energy. When the pyoverdin production rate reached its maxi mum, the demand of iron-depleted cultures for O-2 was decreased. Mutant FF1 3 overproduced pyoverdin in both iron-depleted (862 mg l(-1)) and iron-supp lemented (428 mg l(-1)) CA medium and could also be used to produce pyoverd in when grown in a conventional stirred tank fermenter.