A. Johnova et al., Overproduction of pyoverdins by Fur mutants of Pseudomonas aeruginosa strains PAO1 and Fe10 in stirred bioreactors, BIOTECH LET, 23(21), 2001, pp. 1759-1763
Fur mutants FPA12 and FF13 of strains Pseudomonas aeruginosa PAO1 and Fe10,
respectively, were prepared and their production of pyoverdin evaluated. T
he strains were cultivated in stirred bioreactor in iron-deficient and iron
-supplemented medium containing Casamino acids (CA) or succinate as a sourc
e of carbon and energy. When the pyoverdin production rate reached its maxi
mum, the demand of iron-depleted cultures for O-2 was decreased. Mutant FF1
3 overproduced pyoverdin in both iron-depleted (862 mg l(-1)) and iron-supp
lemented (428 mg l(-1)) CA medium and could also be used to produce pyoverd
in when grown in a conventional stirred tank fermenter.