Cloning and sequence analysis of bile salt hydrolase (bsh) gene from Bifidobacterium longum

Authors
Shuhaimi, M Ali, AM Saleh, NM Yazid, AM
Citation
M. Shuhaimi et al., Cloning and sequence analysis of bile salt hydrolase (bsh) gene from Bifidobacterium longum, BIOTECH LET, 23(21), 2001, pp. 1775-1780
Citations number
19
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
BIOTECHNOLOGY LETTERS
ISSN journal
01415492 → ACNP
Volume
23
Issue
21
Year of publication
2001
Pages
1775 - 1780
Database
ISI
SICI code
0141-5492(2001)23:21<1775:CASAOB>2.0.ZU;2-F
Abstract
A pair of PCR primers for the rapid detection of bile salt hydrolase (bsh) gene from Bifidobacterium longum BB536 has been synthesised and have reveal ed the bsh gene of approx 970 bp in Bifidobacterium longum BB 536 but not i n other species of bacteria tested. The bsh gene was cloned and sequenced s howing a high similarity to bsh gene previously published. The resulting nu cleotide sequence encodes a predicted protein of 317 amino acids, Mw = 35 k Da.