Bg. Blijenberg et al., Discordant performance of assays for free and total prostate-specific antigen in relation to the early detection of prostate cancer, BJU INT, 88(6), 2001, pp. 545-550
Objective To assess the value of applying rigid threshold values in interpr
eting prostate specific antigen (PSA) results, by selecting and comparing f
ive current methods for measuring free and total PSA.
Materials and methods Samples taken from an ongoing screening study for pro
state cancer (total PSA by Tandem-E assay, 17 334 participants; biopsy crit
erion a PSA of 3.0 mug/L, 4 464 men) from men with a total PSA of 1.0-6.0 m
ug/L were measured for free and total PSA using the Access, Immulite, Elecs
ys and Prostatus analysis kits, in two patient groups, i.e. with prostate c
ancer or no evidence of disease.
Results Both patient groups had equal means for total PSA but not for free
PSA. In all, 360 samples from men with cancer and 96 from men with no evide
nce of disease were analysed. All methods applied to both groups deviated s
tatistically significantly from the Tandem-E result for total PSA, except f
or the Access kit. There was a close correlation among all the methods (cor
relation coefficients of 0.89-0.97). There were very discordant results for
the combination of the Tandem-E vs Prostatus (8% difference), representing
315 participants at a threshold of 3.0 mug/L. For free PSA (free/total PSA
) the situation was worse, with extreme differences of 32% and 36% for both
patient groups (Elecsys vs Access).
Conclusions Depending on the threshold value applied as an indication for b
iopsy, when using the total PSA alone or combined with the free/total PSA,
care is needed in interpreting patient groups because of the discordance am
ong PSA assays.