Differences in the laminar origin of projections from the medial prefrontal cortex to the nucleus accumbens shell and core regions in the rat

The medial preftontal cortex (mPFC) projects to the nucleus accumbens shell , core and rostral pole. In this retrograde tract-tracing study of rat mPFC to nucleus accumbens projection neurons, the advantages of Neurobiotin are utilised in order to reveal the detailed morphology of labelled projection cells, and to permit an examination of the laminar projections to shell an d core compartments The retrogradely transported Neurobiotin was found in s omata, proximal and distal dendrites of neurons that project from the mPFC to the nucleus accumbens. The morphology of these projection neurons was re vealed in great detail and confirmed that the projection arises wholly from pyramidal cells. Interestingly, it was also found that retrogradely labell ed neurons were exclusively located in prelimbic and infralimbic regions in layers V and VI, after shell injections, but also in layer II following co re sites. This observation may reflect possibly different roles for cortica l laminae on the nucleus accumbens. (C) 2001 Elsevier Science BY All rights reserved.