An alkali stable cellulase by chemical modification using maleic anhydride

Authors
Bund, RK Singhal, RS
Citation
Rk. Bund et Rs. Singhal, An alkali stable cellulase by chemical modification using maleic anhydride, CARBOHY POL, 47(2), 2002, pp. 137-141
Citations number
13
Categorie Soggetti
Agricultural Chemistry","Chemistry & Analysis","Organic Chemistry/Polymer Science
Journal title
CARBOHYDRATE POLYMERS
ISSN journal
01448617 → ACNP
Volume
47
Issue
2
Year of publication
2002
Pages
137 - 141
Database
ISI
SICI code
0144-8617(20020201)47:2<137:AASCBC>2.0.ZU;2-M
Abstract
An alkali stable cellulase was obtained by chemical modification with 0.5 M maleic anhydride at 2-4 degreesC and pH 8.0 for 25 min. The cellulase so m odified showed a marginally better activity in alkaline range as compared t o native cellulase. It had a half-life of 462.0, 433.13, 128.33 and 144.38 h vis-h-vis 13.08, 121.58, 126.0 and 187.30 for native unmodified enzyme at a pH of 8.0, 9.0, 10.0 and 11.0, respectively, at 50 degreesC. These resul ts could have promising applications in textiles and detergents. (C) 2002 E lsevier Science Ltd. All rights reserved.