HPLC with evaporative light scattering detection for the determination of polar compounds in used frying oils

Authors
Kaufmann, A Ryser, B Suter, B
Citation
A. Kaufmann et al., HPLC with evaporative light scattering detection for the determination of polar compounds in used frying oils, EUR FOOD RE, 213(4-5), 2001, pp. 372-376
Citations number
17
Categorie Soggetti
Food Science/Nutrition
Journal title
EUROPEAN FOOD RESEARCH AND TECHNOLOGY
ISSN journal
14382377 → ACNP
Volume
213
Issue
4-5
Year of publication
2001
Pages
372 - 376
Database
ISI
SICI code
1438-2377(200110)213:4-5<372:HWELSD>2.0.ZU;2-U
Abstract
An HPLC method for the determination of polar compounds in used frying oils /fats is described. It is based on normal phase liquid chromatography, i.e. , the same separation mode as the reference method. The use of a mass-sensi tive evaporative light scattering detector (ELSD) permits the quantificatio n of the polar and non-polar compounds under two symmetrical, baseline-reso lved peaks. A polarity gradient is used for the elution of the polar oil co mponents. The results correlate well with the official reference method (r( 2)>0.97). Simplicity, speed, and low consumption of organic solvent are the main advantages.