KINETICS OF ADENOVIRUS-MEDIATED GENE-TRANSFER TO HUMAN LUNG FIBROBLASTS

Many features of Ad-mediated gene transfer to human lung fibroblasts a re not well understood. We tested kinetics of transduction efficiency of LacZ gene to human lung fibroblasts by El-deleted adenoviruses cont aining two different promoters, i.e. CMV and RSV LTR. A dose-dependent relationship between the vector multiplicity of infection (moi) and t he efficiency of LacZ gene transfer to fibroblasts was observed with e ach vector, and higher moi of vectors achieved 100% of transduction ef ficiency. Further, Ad-mediated gene transfer was enhanced by a long pe riod of vector exposure to fibroblasts up to 6 hours. There were no di fferences in transduction efficiency between the two Ad vectors. LacZ gene expression by Ad vectors consistently decreased one day after inf ection. These results indicate that both Ad vectors are equally effect ive for gene transfer to human lung fibroblasts, and higher moi of vec tors and/or a longer period exposure of fibroblasts to vectors can fas cilitate more efficient transduction of LacZ reporter gene into human lung fibroblasts.