Abnormalities in the cell cycle are associated with tumorigenesis but have
not yet been identified in squamous cell carcinoma (SCC) of the vulva or in
adjacent vulvar lesions. The purpose of this study was to identify cell cy
cle protein expression (cyclin D1 and retinoblastoma protein [pRb]) in vulv
ar SCC and in adjacent potentially premalignant lesions: lichen sclerosis (
LS), squamous cell hyperplasia (SH), and vulvar intraepithelial neoplasia (
VIN). Using immunohistochemical techniques, 57 SCCs were analyzed with 19 a
djacent areas showing LS, 13 showing SH, 11 VIN, and six normal epithelium.
Fifty-one percent of SCCs showed abnormal cyclin D1 expression and 37% sho
wed abnormal pRb. Abnormal cyclin D1 expression in the adjacent areas was a
s follows: 53% in LS, 31% in SH, 18% in VIN, and 0% in normal. Abnormal pRb
expression was as follows: 42% in LS, 62% in SH, 46% in VIN, and 33% in no
rmal. Only 10 lesions showed abnormal expression of both proteins. Abnormal
expression of cyclin D1 in SCC was statistically significant compared with
adjacent normal epithelium. In SCC lesions, abnormal cyclin D1 expression
was associated with greater depth of invasion. Abnormal pRb in SCC was asso
ciated with poor tumor grade. Cyclin D1 and pRb are separately involved in
the progression of vulvar cancer, and changes in the expression of these pr
oteins may represent an early stage of malignant transformation in vulvar d
isease.